This paper was focused on changes in contents of trace elements in greenhouse soils under vegetable cultivation for 0 (CK), 1, 5, 8 and 11 years. The results showed that: Except Mo, there were little differences observed in Co, Cu, Fe, Mn, Ni and Zn contents from depth to depth within 0-40 cm; trace element contents decreased significantly in the first year after the field was turned into greenhouse for vegetable cultivation, but they increased significantly afterwards and reached a relative balance after five years; certain positive linear correlations were somewhat found among Co, Cu, Fe, Mn, Ni and Zn contents; large amounts of poultry dung and compound fertilizer were possibly the main sources for the increase in trace element contents in these soil, no soil pollution happened but attention should be paid to the severe deficiency of Mo in the soil.