The results of pot experiments showed that application of Selenium in soil significantly increased Selenium content of radish, and content of organic Selenium in radish root as well. However, higher content of Selenium in soil would decrease the transformation percentage of organic Selenium. It was also indicated that addition of Selenium affected quality of the radish, by increasing the total content of the essential amino acids, but decreasing the total content of amino acids, crude protein, fat and soluble saccharide in radish root, and simultaneously, inhibiting absorption of Cu and Zn.