With the aid of GPS, 9340 soil samples were collected from 50 major tobacco-growing counties of Guizhou province and analyzed within 3 years. The results indicate that tobacco-growing soils in Guizhou are rich and vary sharply in organic matter content and total nitrogen content, which averages out 29.6g/kg and 1.75g/kg, respectively. And soil samples with organic matter content and total N content in the most suitable range account for 48.4% and 64.1%, respectively. The soil samples with available nitrogen contents in the most suitable range account for 42.4%, averaging 50.6mg/kg. Meanwhile, paddy soils are high and purple soils low in both the organic matter content and total nitrogen content and tobacco-growing paddy soil is significantly higher than upland soil in total nitrogen.