采集第四纪红色粘土发育和第三纪红砂岩发育的红壤,分别在4℃冰箱内保存(O),室温下湿润(M)和淹水(F)培养110天后测定硝化细菌、反硝化细菌、硝化势、反硝化势和反硝化酶活性。结果表明,低温有利于保持硝化细菌和反硝化细菌的数量,但显著抑制它们的硝化和反硝化活力。湿润有利于保持硝化细菌的硝化活力,而淹水则有利于保持反硝化细菌的反硝化活力,但均不利于硝化细菌和反硝细菌的存活。由此说明,不同的研究目的和需要测定的项目,应采用不同的土壤样本保存方法。

Two red soils derived from the quaternary red clay and the tertiary red sandstone, respectively, were used to investigate effects of moisture and temperature on nitrification and denitrification activities of the soils. Nitrifiers, denitrifiers, nitrification intensity, denitrification potential and denitrifying enzyme activity were determined of the fresh soil samples having been kept placed at 4℃ (O), moistened to 40% in water holding capacity at room temperature (24℃~27℃) (M) and flooded (F) at room temperature (24℃~27℃), separately for 110 days. The results indicate that low temperature helped keep the number of nitrifiers and denitrifiers, but significantly inhibited nitrification and denitrification activities of the bacteria. Moistening contributed to maintenance of nitrification activity of the nitrifiers. Flooding, though, favored denitrification activity of the denitrifiers, it made againstsurvival of both the nitrifiers and denitrifiers. Therefore, the appropriate way to store soil samples depends on what they will be used for.

国家973项目(G1999011805)和中国科学院研究生科学与社会实践创新研究基金资助。