SOLUBLE ORGANIC C CONTENT OF PADDY SOILS IN SUBTROPICAL CHINA IN RELATION TO EXTRACTION CONDITIONS
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摘要:
通过室内实验研究了不同提取条件对红壤水稻土溶解有机C量的影响。结果表明,浸提时样品的水分含量越高,溶解有机C量越大,特别是水分含量超过600 g/kg时,变化的趋势更明显。水土比也对溶解有机C量有影响,随水土比增大,溶解有机C量升高,水土比>2时,增加的趋势更显著。提取时间的长短对溶解有机C量影响不明显。不同的提取剂种类和浓度对溶解有机C量的影响显著,较高浓度的盐溶液对有机C的溶出作用明显,显著增加溶液中的有机C量。有鉴于此,为使研究结果可以比较,提取的方法应当统一,根据试验结果,认为在进行溶解有机C含量变化研究时,所采取的提取方法应该是:①采用鲜土:含水量在200~400 g/kg;②用去离子水作为提取剂;③提取时的水土比应为2:1;④振荡提取时间为30 min;⑤离心20 min (4000 r/min);⑥用0.45 μm滤膜过滤;⑦滤液中有机C用TOC分析仪测定。
Abstract:
Laboratory analysis was conducted to investigate soluble organic C content of paddy soils in subtropical China in relation to extraction conditions. Results showed that the concentration of water-soluble organic carbon (WSOC) increased with soil moisture, which was more obvious in soil higher than 600 g/kg in moisture content. WSOC was positively and significantly related to water-soil ratio, especially when the water-ratio was over 2. WSOC was not influenced by duration of the extraction when it was not longer than 5 h. Type and concentration of the extraction reagents influenced significantly the soluble organic carbon contents in soils, indicating a higher concentration of extraction reagent would bring out a higher WSOC. Based on the above findings, it can be concluded that to keep experiment results comparable, it is advisable to adopt a uniform extraction method, that is, ①fresh soil samples, 200~400 g/kg in soil moisture content; ②deionized water as extraction reagent; ③water-ratio being 2; ④ shaking and extraction for 30 min; ⑤centrifugation for 20 min (4000 r/min); ⑥0.45 μm membrane for filtration; and ⑦TOC analyzer to determine organic carbon in filtrate.
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李忠佩,焦坤,吴大付.不同提取条件下红壤水稻土溶解有机碳的含量变化[J].土壤,2005,37(5):512-516. LI Zhong-pei, JIAO Kun, WU Da-fu. SOLUBLE ORGANIC C CONTENT OF PADDY SOILS IN SUBTROPICAL CHINA IN RELATION TO EXTRACTION CONDITIONS[J]. Soils,2005,37(5):512-516