Soil fertility is the foundation of the cultivation of Chinese medicinal herbs. Determination of soil fertility of the GAP bases helps provide a scientific basis for reasonable fertilization in cultivation of medicinal herb plants, guarantee prime quality and high yield of Chinese traditional medicinal crops, and meet the requirements of Good Agricultural Practice (GAP) for Chinese Crude Drugs by determination of soil fertility. Routine methods were used to analyze and determine soil nutrients, essential in soil fertility, of five standardized cultivation bases, i.e. Houttuynia cordata Thunb.、Lilium lancifolium Thunb.、Prunella Vugaris L.、Rosa Laevigata Michx. and Senen Lablab Album cultivation bases, located in four counties in Hunan. The results showed that the studied soils were acidic (pH 4.7~5.7), and their contents of organic matter, total nitrogen, ammonium nitrogen, available potassium, and available phosphorus were 22.4~42.4, 1.07~1.58, 6.9~22.8, 67~236 g/kg and 4.2~84.6 g/kg, respectively. Difference did exist between the bases in soil fertility.