Biosurfactant is a high surface-active agent synthesized by microorganism. Compared with chemical surfactant, it has a low toxicity to ecological system and can be easily decomposed. So, it has widely potential uses in industry and researches on its synthesis are becoming more and more widely. As biosurfactant is a secondary metabolizing product, traditional methods can't access the processing easily, so they focus mainly on screening high productive strains and the effects on production by various fermentation conditions. During recent years, with the development of molecular biology, more methods like DNA recombination, DNA shuffling and site-directed mutagenesis are introduced into the biosurfactant research and it has already furthered the research in those fields such as key enzymes in the synthesis, operators of the biosynthetic genes, the changing of substrates.