A pot experiment was carried out to investigate the effects of application of EDTA and EDDS on solubilization of soil metals (Cu, Zn, Pb and Cd) and uptake by Brassica juncea. The results showed that with the same application rate (3 mmol/kg), EDDS was equally effective in solubilizing soil Cu as EDTA. Whereas, EDDS was less effective in solubilizing Zn、Pb and Cd. These conform to the stability constants of chelates. Hyperaccumulation of Cu in the leaves of B. juncea was achieved in all of application of 3 mmol/kg and 6 mmol/kg EDDS treatments. When EDDS was applied as 3 mmol/kg, there was no significant difference in Cu concentrations in the leaves among application modes (in single shot, in two or four splitting shots). Cu concentrations were much higher in the leaves than in the stems. Cu concentration in the stems increased linearly with that in the soil solution. While, Cu concentration in the leaves showed a parabola relationship with that in the soil solution.