Soil moisture influences release of soil dissolved organic carbon (DOC), which is related closely to mineralization of soil organic carbon. By lab incubation experiment, dynamics of DOC contents under different water/soil ratios and mineralization of soil organic carbon under different moisture regimes during the incubation period were observed to analyze relationship between DOC content and soil organic carbon mineralization. Results indicated a significantly positive linear relationship between DOC content and water-soil ratio; DOC concentration under submergence treatment was significantly higher than that under aerobic treatment (P<0.01) during whole incubation period, and difference increased at beginning but then decreased with incubation time; Daily mineralization under aerobic condition was higher than that under submergence, but difference deceased gradually with incubation time.