Vacuole is a multifunctional and complex organelle in plant cell, which occupies about 90% of total volume of stem and blade cells of mature plants. Nitrate in vacuole accounts for almost 58%~99% of total of plant cell, thus, vacuole is considered as a nitrate storage pool, and more and more attentions have been paid to nitrate accumulation in plant vacuole. However, information in this field is still lack and insufficient due to difficulties in measuring nitrate in plant vacuole. Based on relevant work conducted in recent years, nitrate accumulation in plant vacuolar was discussed in this paper, which including:① Amount of nitrate accumulated in plant vacuole; ② Mechanism of nitrate transportation between cytoplasm and vacuole in plant cell; ③ Nutritive significance of nitrate accumulation in vacuolar; ④ Physiological functions of nitrate accumulated in vacuolar; ⑤ Issues that need further research on vacuolar nitrate accumulation.