Bacteriophages MS2 and φX174 have been used extensively as indicator virus widely in studying virus migration in column and field experiments. Requirements and general procedures for the laboratory propagation of bacteriophages MS2 and φX174 using double layer agar plates at a small scale and high multiplicity infection at a large scale were explained in detail. The optimal time when host bacterium demonstrated log growth for host bacteria of E. coli(ATCC 15597)infected with the bacteriophage MS2 was in the period of 90 ~ 120 min, and for host bacteria of E. coli(ATCC 13706)with the bacteriophage φX174 in the period of 120 ~ 150 min. It was observed that the double layer agar plate method was timeand labor-consuming, while the liquid medium culture of high multiplicity infection method could produce large amount of pure solution of bacteriophage, about 500 ml per one culture, with concentration reaching 10¹¹ pfu/ml and 10⁸ pfu/ml for MS2 and φX174, respectively.