The dynamics and effects of corrosion induced by the typical bacteria, fungi and actinomycetes separated from soils in Karst areas of Southwest China were studied by simulated soil column experiments, and the relationship between microbial carbonic anhydrase (CA) activities and element migration of Ca, Mg and Zn in soil-limestone systems were investigated. The results showed that： 1) Typical bacteria, fungi and actinomycetes used in the experiments had strong influences on limestone corrosion, but there were differences in corrosive effects of limestone among different kinds of microbes, and the surface shape of limestone varied with different kinds of microbes. Among the three kinds of microbes in the simulated soil column experiments, fungi had the most significant corrosive effect, and the next were actinomycetes, while the last were bacteria; 2) The effects of the three kinds of typical microbes on migration of different elements were different. The amount of migration of Zn²⁺ and Ca²⁺ were fungi > actinomycetes > bacteria > CK, and the amount of migration of Mg²⁺ was bacteria > Fungi > Actinomycetes > CK; 3) Temperature could improve soil microbial effects on element migration of Ca, Mg and Zn; 4) There was a positive correlation between the mean activity of carbonic anhydrase (CA) and the total amount of Ca²⁺, Mg²⁺ and Zn²⁺ in leachates of soil columns, which indicated that CA had a certain influence on the migration of these three kinds of elements, and the influence varied with different elements. These above results may provide scientific basis to evaluate the roles and status of different kinds of microbes in biokarst and element migration in Karst systems.