T₄₂ trichoderma sp. and Bs-6 Bacillus subtilis significantly inhibite hyphal growth and spore germination of Fusarium oxysporium and Rhizoctonia solani, which are the main diseases strawberry suffers in continuous cropping. Pot and field experiments were carried out on effect of a complex T₄₂ and Bs-6 bio-preparation in controlling strawberry diseases. Results showed that it significantly enhanced the vegetative and reproductive growths of strawberry, reduced the dead seedling rate from 52.9% to 14.5%, increased the yield by 111%, and significantly improved quality of the fruit in comparison with that in the untreated control.