A pot experiment was carried out to investigate effects of the combined Mn and Pb contamination on growth and Pb and Mn uptake or accumulation of tobacco plants. Results showed that the combined Pb and Mn contamination hindered tobacco growth. When the Pb concentration in the soil was kept at the level of 500 mg/kg, the plant height, root length and root/shoot biomass of tobacco plants decreased, as the Mn concentrations in the soil increased. The biomass of plant height, root length and the output of tobacco decreased with the rise of Mn concentration in the soil and the fall became extremely significant (p < 0.01) when soil Mn reached 2500 mg/kg. Combined Mn and Pb contamination also affected Mn and Pb uptake and accumulation by tobacco. When soil Pb concentration was kept at 500 mg/kg, increasing Mn concentration in the soil, brought up the Mn and Pb concentrations in every part of the tobacco plants. Moreover, the addition of Mn promoted Pb uptake and accumulation in tobacco plants.