Based on the field investigation and laboratory analyses, relationships were studied of activities of four soil enzymes (calatase, invertase, urease, phosphatase) with forms, contents, and distribution of Cu, Zn and Pb in paddy soils of five regions different in landform in Anhui Province. Results showed that the contents of Cu, Zn and Pb in residual and organic forms occupied the biggest proportion in the total in all these paddy soils. The contents of organic Cu, Zn and Pb were obviously higher in the plain and hill regions than in the mountain region, whereas the relative contents of Fe-Mn oxide combined Cu, Zn and Pb were the other way round. In paddy soils, carbonate combined Cu and Zn inhibited the activity of calatase the most significantly, while exchangeable Pb did the activity of urease the most. It is, therefore, feasible to use carbonate combined Cu, Zn and calatase activity as biochemical indicators of Cu and Zn pollution, and exchangeable Pb and urease activity as indicators of Pb pollution of soils.