A single factor experiment with randomized plot arrangement was carried out to study effects of utilization pattern of pasture on soil physical, chemical and biological properties in an artificial fruit-grass ecosystem. Results showed that compared with cleaning tillage, the other three treatments, such as mowing and mulching, mowing and burying and applying livestock manure to the orchard, raised soil water content, decreased soil density, stabilized soil temperature, increased soil nutrient content, population of soil micro-organisms, and activity of soil enzymes. A conclusion was reached after a correlation analysis that soil nutrients were positively related to biological factors, except total P, which was negatively correlated with cellulose-decomposing bacteria, cellulolytic enzyme, and polyphenol oxidase. The relationship between them was significant or extremely significant. A pathway analysis revealed that urease, silicate bacterium and cellulolytic enzyme were the main biological factors contributing to accumulation of organic matter; sucrase was the most important factor affecting readily available nutrients of N, P and K; and catalase, polyphenol oxidase, cellulose-decomposing bacteria only selectively affected accumulation of organic matter and formation of readily available nutrients of N, P and K.