A field trial was conducted in a greenhouse with soil affected by secondary salinization to explore effects of application of oil cake manure on soil available nutrients, water soluble salts and salt composition, and hence to evaluate feasibility of applying oil cake manure to ameliorate soils affected by secondary salinization, and predict possible consequences of the application of a large amount of oil cake manure in the greenhouse soil. Results indicated that most of the soil available nutrients, especially the macro nutrients, water soluble salt content and EC in the 0~20 cm soil layer, were significantly increased, and so were some soil available nutrients and salt ions in the 20~30 cm soil layer, thus further aggravating the risk of soil secondary salinization. Therefore, oil cake application was not a good measure to ameliorate salinized soil. In the greenhouse, its application rate should be rationally decreased to avoid the formation and development of soil secondary salinization.