An experiment using monolith lysimeter including different fertilization levels was conducted to study the nutrient leaching amount of rice-wheat rotation field in Taihu Lake area. Results showed that the amount of NH4+-N, NO3--N and TP leaching in wheat season were 0.36 ~ 0.64, 2.74 ~ 16.42 and 0.05 ~ 0.19 kg/hm2, respectively, accounting for 0.2% ~ 0.4%, 4.8% ~ 8.1% and 0.2% ~ 0.7% of applied fertilizer; the amount of leaching in rice season were 0.36 ~ 1.04, 0.86 ~ 3.01 and 0.24 ~ 1.17 kg/hm2, respectively, accounting for 0.2% ~ 0.4%, 0.6% ~ 1.7% and 0.8% ~ 8.2%. Compared to using fertilizer only, manure combined with fertilizer increased the nutrient leaching, particularly for phosphorus, while straw combined fertilizer reduced the nitrogen leaching from wheat field.