A pot experiment was carried out to study the effects of soil moisture on the growth and heavy metals uptake of Sedum plumbizincicola under different water holding capacities (WHC). The results showed that Sedum plumbizincicola grew best under the treatment of 70% of WHC, its aboveground plant fresh weight was significantly higher than those of other treatments, its heavy metal uptaking amount was also the highest, and Zn concentration in stem was significantly higher than those of others treatments. Cd concentration in stem under 70% of WHC were 27.1%, 29.0% and 63.1% higher compared to 35%WHC, 100%WHC and water logging treatments respectively; Zn concentration in leaf showed the same trend as the stem, however, Cd concentration in leaf didn’t show the same trend as the stem, which showed the highest Cd concentration under 100% of WHC. The phytoextraction efficiency of Zn and Cd were the highest under 70% of WHC. These above results showed that soil moisture plays an important role for the heavy metal phytoextraction by Sedum plumbizincicola.