The spatial variability of organic matter, available N and available K in red soil hilly region of Jinhua County, Zhejiang Province were studied by GIS and geostatistics. The results showed that：The contents of the three soil characteristics all showed good normal distributions after being transformed by square. The theoretical spherical model was accord with soil organic matter, while the exponential model was accord with soil available N and available K. The three soil characteristics all showed moderate spatial dependence, and their contents were influenced by both the intrinsic and extrinsic factors. The three soil characteristics showed different spatial distribution patterns and each nutrient also showed different spatial variability at different direction.