Study on soil redistribution by tillage erosion can reform the irrational farming methods and control sloping farmland erosion. A research on tillage experiment with tracer method on sloping farmland was conducted in karst area of Zhongliang-mountain in Chongqing city, the results showed that the tracer concentration in tracer district decreased after farming, but still remained high level. With the increase of the slope gradient, the tracer concentration in the tracer district decreased and the maximum concentration occurred not only farther from the baseline but also became lower; the shift distance of tracer became longer along the tillage direction. Those above-phenomena were more obvious in hoe or shovel downslope tillage than those of contour tillage and trample-shovel downslope tillage. Taking tillage erosion into consideration, contour-tillage method obviously is superior to downslope tillage, and trampling-shovel method is superior to hoe and shovel in downslope tillage. The impact of tillage erosion on the rocky desertification was also discussed by combining the conditions of natural environment in Karst mountain area in the paper.