A high efficient tomato (Solanum lycopersicum L. cv Zhongshu No. 4) regeneration system with high frequency regeneration ratio, time-saving and good tube seedlings was developed. Detailed protocols were as follows: Hypocotyls were placed on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L zeatin (ZT) and 0.1 mg/L indole-3-acetic acid (IAA) or 2.0 mg/L 6-benzyladenine (BA) and 0.2 mg/L IAA for inducing adventitious buds; after being cultured for 21 d, hypocotyls were sub-cultured to fresh medium once, and then the buds were cut and induced roots on MS medium supplemented with 0.1 mg/L IAA; well-rooting buds were acclimated to greenhouse conditions for 2-3 d, and then transplanted to well-watered vermiculite for 3-7 d, finally transplanted to vegetable garden soil.