一株副球菌对污染土壤中多环芳烃的降解研究
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中国科学院知识创新工程重要方向项目(KZCX2-YW-404, CXTD-Z2005-4)、国家重点基础研究发展规划项目(2002CB410809)和国家“863计划”项目(2007AA061101)资助


Biodegradation of PAHs by Paracoccus aminovorans HPD-2 in Contaminated Soil
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    摘要:

    从受多环芳烃(PAHs)长期污染的土壤中分离到一株降解PAHs的噬氨副球菌(Paracoccus aminovorans)HPD-2。使用HPD-2的菌液对PAHs污染的土壤进行了2周的生物降解试验,结果表明加入HPD-2能够明显提高土壤中PAHs的降解率。加菌土壤中PAHs的总去除率为22.9%,PAHs各组分的降解率在19.5% ~ 36.2% 之间。其中三环PAHs的降解率最高(36.1%),五环次之(26.0%),四环的最低(20.9%)。对土壤微生物的计数结果发现,HPD-2的加入显著提高了土壤中细菌的数量,而对放线菌和真菌的影响不明显。PCR-DGGE分析结果表明,降解过程中HPD-2可能成为土壤中的优势菌。以上结果表明该菌株在PAHs污染土壤的生物降解中具有较好的应用前景。

    Abstract:

    The effects of the inoculant strain Paracoccus aminovorans HPD-2 (isolated from a PAH-contaminated soil) on the bioremediation of the PAHs-contaminated soil and the structure of microbial communities in the soil were studied in soil microcosms. After inoculation for 14 days, 22.9% of total PAHs were removed from the soil in the treatment with the addition of 20% (v/m) bacterium suspension. The removal of 3-rings PAHs (36.1%) was higher than that of 5-rings (26.0%) and 4-rings PAHs (20.9%). The highest degradation of PAHs occurred in phenanthrene, anthracene, and benzo[a]pyrene. Bacterial population in the soil increased during the bioremediation process, but the population of actinomycetes and fungi changed insignificantly. Denaturing gradient gel electrophoresis (DGGE) analysis of polymerase chain reaction (PCR)-amplified bacterial 16S rRNA gene fragments revealed that HPD-2 became into the dominant bacterial group in the soil with the addition of the bacterium suspension. These results indicate that HPD-2 is a promising candidate for bioremediation of PAHs-contaminated soils.

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毛 健,骆永明,滕 应,李振高.一株副球菌对污染土壤中多环芳烃的降解研究[J].土壤,2009,41(3):448-453. MAO Jian, LUO Yong-ming, TENG Ying, LI Zhen-gao. Biodegradation of PAHs by Paracoccus aminovorans HPD-2 in Contaminated Soil[J]. Soils,2009,41(3):448-453

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