Aluminum toxicity and phosphorus deficiency are the most important limit factors for crop growth in acid soils in many regions of the world, the problem is particularly severe when pH is below 5.0. Lespedeza bicolor is a forage legume, well adapted to acid infertile soils. This paper investigated mineral nutrition of Lespedeza bicolor under high Al and low phosphorus stress. The results showed that Al stress significantly reduced Ca content in the shoots and roots and Mg accumulation in the roots; but had no effect on the contents of K, P, Fe, Zn and Cu of two Lespedeza cultivars. Low phosphorus treatment significantly reduced the absorption and transport of Mg in the roots of Hebei Lespedeza cultivars, but had no effect on the contents of K, P, Fe, Zn and Cu of two Lespedeza cultivars. Al accumulated mainly in the roots of Lespedeza bicolor, which indicated Al tolerance of Lespedeza bicolor was achieved by restricting Al absorption from the soils and Al upward transportation of in the plants.