Eleven kinds of bacteria with high capability of accumulating poly-P were isolated and screened from the bottom muds of Chaohu Lake and South Feihe River by using the pure culture and blue and white colored screening methods. Their phosphate accumulating capacities were investigated aerobically in synthesized sewage medium. The results showed that the total phosphorus in the GP44 cells was 11.92%, indicating a higher level of phosphorus accumulation, and it was identified as K.terrigena. GP44 grew in synthesized sewage medium with the optimal phosphate accumulating temperature, initial pH value, aeration ration and carbon source was 30℃, 7.5, 120 ml/250 ml and glucose respectively, and Mg2+, K+ and Fe3+ could promote GP44 to accumulate phosphorus.