Greenhouse cultivation has become one of the important cropping systems in Yunnan Province. The accumulation of salt caused by different fertilization may affect soil ecosystem in Greenhouse. This paper investigated salt contents in surface soil horizon in greenhouses from six major areas in Yunnan Province. The results showed that: the mean content of soil salt was 1.76 mg/kg, varied in a range of 0.50 - 4.22 mg/kg. Soil salt content and EC value changed obviously in different areas. With the increase of the greenhouse age, salt content and EC values of soil increased. Soil salt accumulated mainly in the top horizon, and decreased gradually with soil depth. Na+ is not the decisive factor for soil salinization.