Antibiotic bacterial fertilizer of Bacillus sp. A03 was employed when flue-cured tobacco transplanted in plots. Rhizobacterial amount considerably increased, while rhizo-actinomycetic amount significantly reduced, rhizo-fungic amount did not alter with antibiotic bacterial fertilizer compared to the control. In addition the resistance of tobacco against tobacco virus enhanced; the availability of 6 mineral elements in soil at tobacco toping stage increased by 16.57% - 56.39% except Ca and Mg, whose availability slightly decreased; the contents of N and P in de-enzyme and tobacco leaves at different growth stages and flue–cured tobacco leaves were higher; the yield and production value per 0.067ha with antibiotic bacterial fertilizer were 10.45% and 11.22% higher respectively compared to control. Furthermore first-class tobacco leaves proportion and mean price with antibiotic bacterial fertilizer were higher than those of control and commercial bacterial fertilizer.