Rhizobag pot experiment was carried out to compare Se accumulation ability in rice seedlings between colored (Longqing4 and Heimi120) and general (Xiushui48 and S.Andrea) rice cultivars, and the correlation between Se accumulation ability and rhizosphere characteristics. The results showed that Se accumulation ability in rice seedlings did not correlated with rice grain color, but correlated with the ability of transporting Se to shoots from roots. Xiushui48 and Longqing4 had higher capacity to accumulate Se in shoots at different Se treatments. General rice cultivars had higher response to Se application. Se-enriched rice varieties also had greater capacity of uptake and accumulate N, P in shoots, and the order was: Xiushui48 > Longqing4> Heimi120 > S.Andrea. Correlation analysis indicated that there were significant correlations between shoot Se and shoot P or shoot N contents. No correlation occurred between shoot Se and shoot K or rhizospheric pH. Higher (shoot Se)/(root Se) ratio might be one of the reasons for Se-enrichment in both colored and general rice.