The article aims to introduce the application of the P-S-R model and Multi-objective and multilevel fuzzy optimization model to evaluate the eco-environmental security of Jinta Oasis agriculture. Based on ecological security theory and P-S-R indicator system model, a 4-level indicator system for Jinta Oasis agriculture was established, which includes the pressure of eco-environmental, the state of eco-environmental and the response of eco-environmental. On this basis, the fuzzy optimization model for evaluation of Jinta Oasis agriculture eco-environmental security was established upon the multi-objective and multilevel fuzzy optimization theory, then, its agriculture eco-environmental security from 1988-2007 was evaluated, the results showed that the general level of eco-environmental security is poor, eco-environmental deteriorates continuously, the serious situation of eco-environmental security is becoming more unfavorable for the existence and development of human being.