Both organic fertilizer application and water management are the most important factors that affect CH4 emission from rice field. Incubation and field experiment were carried out to study the effects of rice straw application in winter on CH4 production, oxidation, and emission from continuously-flooded rice field during the rice-growing season. Results showed that significant difference was observed between the Treatments flooded with and without rice straw incorporation (FS and CK) in CH4 production potential after rice transplanting 35 and 51 days (p＜0.05), while no significant difference was observed in the rest of rice period (p＞0.05). The soil temperature and N-fertilizer application were the more important influencing factors than rice straw application in winter which did not significantly affect CH4 oxidation potential during the rice growth season (p＞0.05). Compared with the treatment CK (CH4 20.3 mg/(m2·h)) in the mean CH4 flux, the treatment FS (CH4 26.7 mg/(m2·h)) was significantly higher (p＜0.05).