In this study, we took three natural slopes (Sunny slope, East slope and West slope) in a small watershed of Shandong Province to explore the spatial variability characteristics of soil nutrients. The results showed that organic matter content was in a trend of East slope> West slope > Sunny slope when the distance was less than 20 m along the slope direction but in a trend of West slope > Sunny slope > East slope when the distance was more than 30 m. Along the slope direction, soil organic matter and available N, P and K contents of the three slopes were different, and were in a general trend of lower in the upper and higher in the bottom of the slopes. Soil organic matter and available N, P and K contents of East slope and West slope decreased as the depth increased. The average content of organic matter was about 8 g/kg at 0-20 cm and about 2 g/kg at 80-100 cm and available N content was 50 mg/kg at 0-20 cm and 20 mg/kg at 80-100 cm.