The effects of distribution of Cu forms, soil enzymatic activity and amount of microbial were studied in a field experiment after applied with apatite, lime, charcoal, pig manure and iron powder in Cu polluted soil. The results showed that percent of exchange state Cu decreased, percent of carbonate combined state Cu, Fe-Mn oxide combined state Cu and organic combined state Cu increased, but did not influence significantly residual state Cu. The activities of hydrogen peroxidase and acid phosphatase increased with apatite, lime, charcoal application, while urease activity changed little. The amount of bacteria and fungi increased after amendments applied, and positive correlation existed between the amount of microbial and soil pH, especially for fungi, the correlation coefficient reached 0.90 (p＜0.05). Soil pH is the main factor influenced distribution of Cu forms, soil enzymatic activity and amount of microbial. Apatite, lime and charcoal not only increased soil pH, decreased the activity of Cu in soil, but also enhanced the amount of microbial and soil enzyme activity, and then were considered as the most favorable remediation for Cu contaminated soil.