Growth chamber hydroponics and glass house pot experiment were conducted to study the tolerance and uptake of Pb in hydroponics condition and of Cu, Pb, Zn and Cd under mixed heavy metal contaminated conditions by Potentilla supina from a Cu mine area, the results showed that Potentilla supina could grow normally with the increase of Pb concentration in hydroponics solution, toxic symptom such as slow plant growth with yellow leaves and black roots at the treatment of high Pb concentration, but plant did not die, Potentilla supina had strong tolerance to Pb at hydroponics culture. Pb concentrations reached to the maximum of 947 mg/kg and 71 053 mg/kg in shoot and root at the highest concentration of solution Pb 3 600 mol/L. In pot experiment, plant grew well in the treatment of control, but grew slowly at the mixed metal pollution soils, Cu, Zn, Pb and Cd in the shoot were 741 ± 164, 18 248 ± 2 222, 1 543 ± 483 and 29.4 ± 5.2 mg/kg respectively at the treatment of Cu 200 mg/kg, Zn 1 000 mg/kg, Pb 1 000 mg/kg and Cd 5 mg/kg, but plant died at the treatment with very high metal pollution level. These results showed that Potentilla supina has strong tolerant to Pb and Cd, Zn, Cu co-contamination, and can be used to remediate heavy metal polluted soil.