A pot experiment in greenhouse was carried out to compare the effects of the application level of fermented dung (0.33%, 0.50% and 1.00%) on soil spore density, root mycorrhizal colonization, plant biomass and root-shoot ratio as well as the P-uptake efficiency of Sorghum sudanense grown in a sterilized soil inoculated with arbuscular mycorrhizal fungus (AMF) Glomus caledonium. AMF inoculation tended to decrease aerial biomass of Sorghum sudanense, but significantly elevated (p<0.05) the root-shoot ratio. The application of 0.33% and 0.50% fermented dung tended to increase soil spore density, root biomass and mycorrhizal colonization of Sorghum sudanense, and significantly increased (p<0.05) the P-uptake efficiency of the root system, while did not alter shoot-root ratio. However, the application of 1.00% fermented dung significantly accelerated (p<0.05) soil spore density, root biomass and mycorrhizal colonization of Sorghum sudanense with the same shoot-root ratio and 1.83 times of the P uptake efficiency as much as that inoculated with AMF alone. It suggests that the application of 1.00% fermented dung is the proper level for the propagation of Glomus caledonium and the elevation of P-uptake efficiency of Sorghum sudanense.