Overall planning of land use division is the key content of general land use planning revision at provincial level. According to the requirements of the strategy for regional development policy and socio-economic development of Tianshui City, the regional land use divisional comprehensive evaluation index system was built up by selecting 20 indexes from the following 5 fields of regional land use: economic development, social development, environment, land utilization situation and benefit. Comprehensive evaluation of land use extent taking counties and cities as units in Tianshui was made by using principal component analysis. Combined with clustering analysis, Tianshui was divided into 3 land-use zones, and some countermeasures and advices for land-use strategy and policy were put forward. Furthermore, based on land suitability evaluation, Tianshui was divided into 5 regulation zones using GIS spatial analysis, combined with data of DEM, slope, soil, temperature and precipitation, and the regulation measures were suggested for land utilizations in various zones. This study can provide scientific basis for regional land use planning, eco-environment protection and construction planning.