Black carbon material was produced by charring the rice straw under the different temperatures (350℃, 600℃ and 850℃, respectively) and was mixed with soil, and incubated for 112 days at 15℃ and 25℃. The results indicated that soil CO2 releasing rates under different treatments were more quick at the earlier stage of incubation, and became slower in the later phase. Soil CO2 releasing rates at 15℃ and 25℃ were decreased with incubation time, and the relative CO2 releasing rates were increased with incubation temperature. Under the same temperature, the accumulation amount of soil CO2-C changed with the following order of 350℃＞600℃＞850℃. Soil organic carbon mineralization rates had significant differences due to black carbon application, indicating that black carbon derived from different thermal temperatures had markedly influenced the mineralization of soil organic carbon.