The Effects of different applying methods of Cu and Zn on the growth, enzyme (PPO and POD) activities and tanshinone of cultivated Salviae Miltiorrhizae Bunge were studied under the condition of soil culture. The results showed that three applying methods (base application, topdressing and spraying application) of interactive application of Cu and Zn were better than those of separate application of Cu or Zn. Three applying methods of Cu alone promoted significantly the growth and accumulation of tanshinone of Salviae Miltiorrhizae Bunge. Among three applying methods, spraying application was significantly better than the base applying and topdressing and there was no significant difference between the base applying and topdressing. The results revealed that the tested soil had a potential shortage of Cu. In the treatments of Zn alone treatments and the interactive application of Cu and Zn, topdressing treatment was best of all, base application was better than spraying application, and tanshinone contents in plant roots in the treatments by different applying methods were statistically significantly different. Compared with CK treatment, spraying on the leaves with Zn solution alone or with Cu plus Zn solution could remarkably increase tanshinone contents in roots. The results indicated that spraying application on the leaves is an effective way to improve the nutritional status of plants, although spraying application couldn’t completely change the nutritional condition of soil deficiency.