In this paper we studied soil nutrients and 5 kinds of enzyme activities in different using intensity units in Inner Mongolia desert grassland, and analyzed the correlations among them. The results showed that using intensity directly affected soil nutrient contents and enzyme activities in desert steppe. Light using activity increased the activities of enzymes and the contents of soil organic matter, total nitrogen and total phosphorus, but heavy using one decreased significantly the activities of catalase and protease activities and the contents of soil organic matter, total phosphorus, total nitrogen and potassium. The activities of urease and polyphenol oxidase varied more in annual quarter. Correlation analysis showed the highest correlation occurred between protease activity and nutrient contents.