Under the lab simulated conditions, the bioremediation of Fungi Stain HQ-C-01 on carbofuran-contaminated soil and the factors affecting the degradation capacity were studied, meanwhile the influences of carbofuran and Fungi Stain HQ-C-01 on soil microflora were also investigated. The results showed that there was positive correlation between the degradation rate and the inoculation quantity. The degradation rate for 50 mg/kg carbofuran after 10 days was 82.89% with 2.09×108 CFU/g inoculation quantity. The degradation rate was low if the inoculation quantity was less than 106 CFU/g. The effect of soil moisture on the degradation rate was obvious, the degradation rate was best (85.32%) when soil moisture was 600 g/kg, but was lower when soil moisture lower than 200 g/kg. Fungi Stain HQ-C-01 had higher degradation rate when the temperature ranged from 25°C to 35°C. Soil pH value influenced the degradation rate significantly, the degradation rate for 50 mg/kg carbofuran after 10 days reached highest (85.629%) when soil pH value was about 7. Carbofuran has certain influence on soil microflora by intensively stimulating soil fungi, but the application of Fungi Stain HQ-C-01 can remit the influence and thus can remediate the contaminated soil.