By using field cell test method, under the condition of applying the same amounts of fertilizer of nitrogen, phosphorus and potassium, this paper studied the effects of different kinds of fertilization treatments (no fertilization, chemical fertilizer, cattle manure + chemical fertilizer, organic and inorganic compound fertilizer) on dynamic changes of soil enzyme activities and soil nutrition supplying abilities during tobacco growing season. The results showed that fertilization significantly increased the soil enzyme activities and soil nutrition supplying abilities during the tobacco growing season except for using cattle manure which decreased hydrogen peroxidase activity. The effects of fertilizing organic and inorganic compound fertilizer was the best, which not only improved soil enzyme activities and nutrition supplying abilities, but also made the changes of urease, acid phosphatase, available nitrogen and available phosphorus of soil consistent with the fertilizer need of flue-cured tobacco; using chemical fertilizer can increase the enzyme activities and soil nutrient supplying abilities in the early stage while the effect of cattle manure + chemical fertilizer appeared in the later stage. Correlation analysis showed that urease and acid phosphatase activities could reflect the changes of soil nutrient contents, thus can be used as biological indicators of soil nutrition supplying ability.