In the present paper, we first discussed the factors that influenced the rice root bleeding collecting by using absorbent cotton absorbing methods. It can be concluded that: it was best to use 2.0g absorbent cotton to collect the bleeding during the heading stage of Liangyoupeijiu (LYPJ); there was significantly difference between LYPJ’s stem and the tiller（P＜0.05）; the bleeding intensities were different significantly during the day and night time（P＜0.01）, the bleeding intensity was higher at day than at night; the rice stake height did not influence the bleeding intensity（P＞0.05）; there was no difference between the first time and the second time of mowing rice stake（P＞0.05）. Under the direction of above methods, we studied the LYPJ’s roots bleeding change after its heading by using Shanyou63 as CK. It was concluded that: about one week after their heading, the root vigor (roots bleeding intensity) of LYPJ and its referring variety Shanyou63 all reached the highest peak, then declined. Compared with the Shanyou63, the vigor of LYPJ declined more seriously and kept at lower level.