Triton X-100, Tween-80 and HPCD were added to an aged agricultural soil to prepare spiked soil under different concentrations, and Tenax-TA was used to extract polycyclic aromatic hydrocarbons from the spiked soil to assess the PAHs bioavailability to earthworm (Eisenia Fetida). The results showed that surfactants could significantly enhance the concentration of the PAHs in Tenax-TA extraction and earthworm accumulation. Compared with the control experiment, PAHs concentration by Tenax-TA extraction and earthworm accumulation in the presence of surfactant increased by 42.3% - 269% and 10% - 340% respectively. Surfactant-enhanced remediation of contaminated soil might release the solidified and stabilized PAHs into the environment and increase the environmental risk.