Pot experiments were carried out to investigate the effects of two new bio-organic fertilizers (BIO-36 and BIO-23) fermented with different antagonistic strains on plant growth and the suppression on the disease. The results were as follows. (1) Application of BIO-36 and BIO-23 significantly increased fresh weights of eggplant seedlings after grown in nursery cups for 30 days. BIO-36 showed most distinct root growth enhancement, being 8.48 to 8.69 times higher in total root length, 2.70 to 2.77 times higher in total surface root area, and 15.5 to 18 times higher in root volume than control. (2) Plant height, shoot and root biomass in the treatments of BIO-36 and BIO-23 were significantly increased after grown for 35 days in the big pots, in which soil inoculated with the pathogen and seedlings were transplanted from nursery cups, while disease incidence of the bacterial wilt was significantly reduced to 4.1% for BIO-36 and 8.3% for BIO-23 in contrast to 91.7% for control. (3) BIO-36 and BIO-23 increased rhizosphere fungi by 4.1 to 4.6 and 3.89 to 4.64 times and rhizosphere actinomycetes by 3.38 to 3.78 times and 3.08 to 3.47 times, respectively, as compared with control, but did not significantly change bacterial counts. However, BIO-36 and BIO-23 reduced the counts of Ralstonia solanacearum in rhizosphere by almost one order of magnitude when compared with control. Therefore, the application of the bio-organic fertilizers could effectively control the bacterial wilt disease and promote plant growth of eggplant.