A DSE stain was isolated from plant roots of Arundinella bengalensis (Spreng.) Druce, naturally growing on the plot of lead-zinc mine areas in Huize, Yunnan Province. The isolate was identified as an Exophiala pisciphila strain by morphological characteristics and ITS1-5.8S rDNA-ITS 2 sequence analysis. The tolerance to heavy metals and accumulation abilities of the E. pisciphila strain were investigated. MICs (minimum inhibitory concentration) to Pb2+, Zn2+ and Cd2+ of the fungus were 2.3, 0.51, and 3.1 mg/ml, respectively. The E. pisciphila strain was able to accumulate lead, zinc and cadmium over 20%, 15% and 5% of its biomass (by dry weight), respectively. It was demonstrated that the fungi was supper accumulative and multi-tolerant to these metals. Fungi colonization in the roots may play important roles in reducing toxicity to the plant root cells of heavy metals in soils.