In order to discuss the effects of a great quantity nitrogen fertilizer application on calcium status of soil, based on long-term experiment located soil in protected field, this study systematically investigated the effects of single nitrogen fertilizer and nitrogen fertilizer match with manure application on calcium distribution of soil. The results indicated that: the contents of total and water soluble calcium distribution in single nitrogen fertilizer and match with manure application soil were higher in 0-20 cm than in 20-40 cm, and adsorption calcium was less in 0-20 cm than in 20-40 cm. In two soil layers the content of water soluble calcium was higher in single nitrogen fertilizer application soil than that in match with manure application soil, and the contents of total calcium and adsorption calcium were less than that of match. Total calcium distribution of different particle sizes was in order of (＜0.1 mm）＞0.1-0.2 mm＞0.2-0.5 mm＞0.5-1 mm in single nitrogen fertilizer application soil and was in order of (＜0.1 mm）＞ 0.5-1 mm ＞0.1-0.2 mm ＞0.2-0.5 mm in nitrogen fertilizer match with manure application soil. Water soluble calcium and adsorption calcium distribution of different particles sizes were in order of (＜0.1 mm）＞0.1-0.2 mm＞0.2-0.5mm＞0.5-1 mm in two groups of treatment soil.