Abstract:In this study, three types of soil and flue cured tobacco of Yanbian area with randomized block design in the field were used to investigate the activities of catalase, urease and invertase, and the contents of alkali-hydrolyzable N, available P and K in rhizosphere and non- rhizosphere soils, and pH values, soil enzymes and nutrients at different developmental stages of tobacco plant. Results indicated that the average activities of catalase, urease and invertase in rhizosphere soils for planting tobacco were 0.60U, 0.48U and 22.09U, respectively. The rhizosphere effect of soil enzyme were obvious different, i.e. catalase (R/S = 1.08)<urease (R/S = 1.14)<invertas (R/S = 1.51). pH value and the contents of alkali-hydrolyzable N, available P and K of rhizosphere soils were 5.42, 72.52 mg/kg, 33.36 mg/kg and 402.04 mg/kg, respectively. The rhizosphere effect was in an order of pH(R/S = 0.91)< alkali-hydrolyzable N (R/S =0.98)<available P (R/S =1.99) <available K (R/S =2.46). pH value and the contents of alkali-hydrolyzable N, available P and K in rhizosphere and non- rhizosphere soils were in an order of dark brown soil>black sandy soil>albic Soil. Therefore, dark brown soil had higher rhizosphere effect and could provide more favorable environment including nutrition for planting tobacco, which might be one of the important reasons that dark brown earths was better than other two soils for tobacco growth.