Abstract:In this study, a field experiment was conducted in Jiangyan of Jiangsu Province to study the effects of different fertilizer treatments on yield and seed quality of rape (Brassica napus L.). Ten fertilizer treatments were designed, i.e: Control (no fertilizer, CK), Nitrogen Deficiency Treatment (PKB), Phosphorus Deficiency Treatment (NKB), Potassium Deficiency Treatment (NPB), Macro-nutrients Balanced Fertilizer Treatment (NPK), Micro-nutrients Adding Treatments (NPKB and NPKBZn), Different Nitrogen Fertilizer Application Treatments (1/2N+PKB, 3/2N+PKB, 2N+PKB). The results were showed as follows: 1) Deletion of any kind of nutrient for N, P and K could decrease yield, and grain yield reached the maximum when N fertilizer application rate was 18 kg/667m2. B and Zn had no significant effect on rapeseed yield. 2) In the three nutrient deficiency treatments (PKB, NKB, NPB), glucosinolate content was in the order of NKB>PKB>NPB, erucic acid content in order of PKB>NKB> NPB, oil content presented no significant difference within the three treatments, protein content had no significant difference between NKB and NPB, but higher than PKB. 3) B fertilizer application, under NPK balanced fertilization conditions, could significantly reduce the contents of glucosinolate and erucic acid, and significantly increase the contents of oil, oleic acid and protein. Based on the application of NPKB, adding Zn fertilizer could significantly further reduce the contents of glucosinolate and erucic acid, and significantly increase the contents of oleic acid and protein, although it had no significant effect on oil content. 4) There was an inflection point of glucosinolate content under different N application rate treatments. Erucic acid content significantly decreased with the increasing N fertilizer application rate. Oil content significantly decreased when N fertilizer application was high (2N+PKB). The responses of the contents of oleic acid and protein to different N fertilizer application rates were same, which increased significantly with the increasing of N fertilizer application rates less than 12 kg/667 m2, and then kept stable when N fertilizer application rate continued to increase.