细菌生长过程中的病毒消失行为研究
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国家自然科学基金面上项目(0971129)资助


Effects of Bacterial Growth Process on Virus Removal
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    摘要:

    环境中土著微生物存在及微生物的不同生长阶段可能影响病毒去向。本研究选择自然界广泛存在的3株细菌:恶臭假单胞菌(PP)、铜绿假单胞菌(PA)、枯草芽孢杆菌(BS)为微生物代表,以噬菌体fX174为指示病毒,通过30℃(PP)和35℃(PA和BS)条件下病毒与细菌同步生长的培养实验和4℃条件下病毒与不同生长时段的菌株培养物的静态吸附实验,比较分析了细菌生长发育阶段对病毒消失(包括可逆/不可逆吸附和消亡)的影响。培育实验结果表明,病毒在30℃ 和35℃ 培养基中的浓度随着培育时间持续降低;但当培养基中接菌时,病毒浓度从接种至对数期6 h间急剧降低,降低幅度超过对照处理,其中在BS处理中降幅最大,其次为PA和PP处理;随着细菌由对数期进入稳定期和衰亡期,病毒浓度先反弹升高,然后后持续降低,反弹最高点病毒浓度在3株菌处理中均高于各自对照处理,反弹持续时间和病毒浓度的反弹提升程度依不同菌株而异。静态实验结果表明,菌株培养物的培育时间从6 h延长至18 h时,病毒的消失比例逐渐降低,继续延长至24 h时又迅速升高,然后随着培育时间的进一步延长而持续降低。以上结果表明细菌对病毒消失的影响随细菌生长发育阶段和菌种而异,暗示在研究病毒在环境中去向时,必须考虑环境中本土微生物的存在及其组成。

    Abstract:

    The existence and different growth stages of local microbe in environment may affect the virus fate. In this study, Pseudomonas putida (PP), Pseudomonas aeruginosa (PA) and Bacillus subtilis (BS), widely found in nature, were chosen as microbe representative. Using bacteriophage fX174 as virus indicator to do synchronization culture experiments with the bacteria under the condition of 30℃ (PP) and 35℃ (PA and BS) and static adsorption experiments with the strain cultures of different growth periods under the condition of 4℃, the impacts of bacterial growth and development stages on virus removal (including the reversible / irreversible adsorption and demise) were compared and analyzed. The incubation experiment results showed that in cultures of 30℃ and 35℃, the virus concentration decreased with the extension of incubation time, but when medium inoculated, virus concentration drastically reduced more than the control from inoculation to the logarithmic phase of 6 h, the largest decline was found in BS, followed by PA and PP. With the bacteria from the logarithmic phase into the stable phase and decline phase, virus concentration rebounded to rise at first and then continued to reduce. To the three bacteria, the highest points of the rebound virus concentration were higher than their control respectively, the duration and extent of virus concentration enhance of rebound varied according to different strains. The static experimental results showed that the proportion of virus removal was gradually reduced when the incubation time of strain culture extended from 6 h to 18 h, but rapidly increased when extended to 24 h and then decreased with further extension of time. These results suggested that the influence of bacteria on virus removal was changed with bacterial growth stages and strains, which implies that the existence of local microbes and their components should be considered when studying the fate of viruses in the environment.

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蒋 艳,赵炳梓.细菌生长过程中的病毒消失行为研究[J].土壤,2013,45(3):522-528. JIANG Yan, ZHAO Bing-zi. Effects of Bacterial Growth Process on Virus Removal[J]. Soils,2013,45(3):522-528

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  • 在线发布日期: 2013-07-24
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