A quadratic regression rotational combination design with four factors, including transplanting density, N, P2O5 and K2O was conducted to study the cultivation models of high yield of pepper Tianjiao No. 9. A mathematical model of relations of pepper yield to transplanting density, nitrogen, phosphorus and potassium was established. Single-factor analysis showed that the yield of pepper increased and then decreased steadily with the increase of the transplanting density, the application of N and P2O5, but it always increased with the increase of the application of K2O. The first order interaction analysis indicated that there existed an interaction range between transplanting density and K2O. The synergistic and antagonistic effects between transplanting density and K2O were shown in the lower and higher range, respectively. The optimizing cultivation models for yield more than 45 000.0 kg/hm2 was calculated by the distributing frequency statistical method. The best transplanting density and amount of fertilizers that obtained yield above 45 000.00 kg/hm2 was: 49 071 to 52 179 holes/hm2 for transplanting density with 2 seedlings/hole, 216.75 to 257.25 kg/hm2 for N, 234.45 to 257.55 kg/hm2 for P2O5, 141.45 to 158.10 kg/hm2 for K2O, respectively. The reasonable proportion of N, P2O5, and K2O in formulated fertilization should be 1.61.61.0. Using the optimizing cultivation, the yield of pepper was increased by 26.6% - 41.3%.