A field experiment was conducted to assess the mitigation of N2O emission using urease inhibitor (Hydroquinone, HQ) and nitrification inhibitor (Dicyandiamide, DCD) under traditional flooding cultivation (TF) and no flooding with plastic mulching cultivation (PM). In the rice growing season, cumulative emission of N2O of treatment TF and PM was 41.8 mg/m2 and 506.9 mg/m2, respectively. The use of HQ/DCD decreased the total N2O emission 25.2% under traditional flooding cultivation and 48.5% under no flooding with plastic mulching cultivation. Under traditional flooding cultivation, total emission of N2O reduced from 0.3% of applied N with urea to 0.2% of applied N with HQ/DCD treatment, and under no flooding with plastic mulching cultivation, the use of HQ/DCD decreased the total emission of N2O from 3.4% to 1.7% of the applied N. Applying the HQ/DCD mitigated the N2O emission more effectively under the no flooding with plastic mulching cultivation compared with the traditional flooding cultivation. No significant correlation was observed between N2O flux, soil Eh or soil temperature in all the treatment.